Project Description

Cryopreservation – General Information

Since the first pregnancy with cryopreserved embryos in 1983, the progress in the field of cryogenics has been rapid and now allows the cryopreservation of the embryos, as well as gametes (eggs and spermatozoa).

The evolution of cryobiology has contributed towards:

  • The reduction in the number of multiple pregnancies, since the excessive number of embryos that can be frozen for use in the next attempt

  • The increase in the overall efficiency of IVF

  • The reduction in the multiple number of IVF cycles, so that the female partner does not have to go through the procedure of egg collection or take medication for ovarian stimulation

New method of cryopreservation: Vitrification

In recent years, great strides have been made in the field of cryobiology with the greatest achievements being the possibility of cryopreservation of male and female gametes (eggs and sperms), as well as embryos and stem cells.

The process of vitrification is a very successful freezing method and is achieved by using high concentrations of cryopreservative substances and the very rapid reduction of the temperature up to -196°C. The cooling rate of eggs -embryos reaches 20.000°C per minute, thus preventing the formation of intracellular water crystals, which causes extensive “damage” inside the cells.

The success rates of vitrification are very high compared to older methods of cryopreservation. It is now possible to freeze eggs with high survival, fertlization and implantation rates after thawing. According to the literature, pregnancies from cryopreserved oocytes can exceed 15%. So far, 300 children worldwide have been born with this method.

With regard to embryos, we expect a significantly higher pregnancy rate per embryo transfer of over 35-40%. The freezing process can take place on the 5th-6th day (blastocyst) with equally high pregnancy rates per embryo transfer of over 27%.


Monday – Friday 8:00 – 13:00
16:00 – 19:00
Saturday 8:00 – 12:00

  + 357 25349960

  + 357 25345550

  + 357 25349950

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