{"id":3364,"date":"2021-02-19T07:26:51","date_gmt":"2021-02-19T07:26:51","guid":{"rendered":"https:\/\/www.iasoivf.com\/?p=3364"},"modified":"2022-11-02T06:20:09","modified_gmt":"2022-11-02T06:20:09","slug":"preimplantation-genetic-testing-pgt-and-ivf","status":"publish","type":"post","link":"https:\/\/www.iasoivf.com\/preimplantation-genetic-testing-pgt-and-ivf\/","title":{"rendered":"Preimplantation Genetic Testing (PGT) and IVF"},"content":{"rendered":"<p><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-1 nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"background-color: rgba(255,255,255,0);background-position: left top;background-repeat: no-repeat;padding-top:100px;padding-right:0px;padding-bottom:75px;padding-left:0px;margin-bottom: 0px;margin-top: 60px;border-width: 0px 0px 0px 0px;border-color:#eae9e9;border-style:solid;\" ><div class=\"fusion-builder-row fusion-row\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-0 fusion_builder_column_1_1 1_1 fusion-one-full fusion-column-first fusion-column-last\" style=\"margin-top:0px;margin-bottom:30px;\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;background-color:#ffffff;border-width: 0px 0px 0px 0px;border-color:#dddddd;border-style:solid;padding: 0px 40px 0px 40px;\"><style type=\"text\/css\"><\/style><div class=\"fusion-title title fusion-title-1 fusion-sep-none fusion-title-text fusion-title-size-three\" style=\"margin-top:0px;margin-bottom:20px;\"><h3 class=\"title-heading-left\" style=\"margin:0;\">Preimplantation Genetic Testing (PGT) and IVF<\/h3><\/div><div class=\"fusion-text fusion-text-1\"><p>Preimplantation Genetic Testing (PGT) is one of the most invasive techniques applied to test oocyte\/embryo genetic content in In Vitro Fertilization (IVF) programs. Embryo biopsy and subsequent molecular analysis are required to detect with high precision the affected embryos in case of inherited monogenic diseases (PGT-M) or in case of numerical\/structural chromosomal defects (PGT-A, PGT-SR).<\/p>\n<p>PGT in IVF cycles leads to significantly reduced chance of pregnancy termination due to genetic disorders and reduced time to achieve a healthy live birth when compared to IVF cycles without any embryo testing. Despite the importance of PGT in IVF programs strict criteria should be implemented to select patients who can effectively benefit from this method.<\/p>\n<p><strong><u>References<\/u><\/strong><\/p>\n<ol>\n<li>Gardner RL, Edwards RG. Control of the sex ratio at full term in the rabbit by transferring sexed blastocysts. Nature, 1968; 218: 346-349.<\/li>\n<li>Saiki RK, et al. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science, 1985; 230: 1.350-1.354.<\/li>\n<li>Li HH, et al. Amplification and analysis of DNA sequences in single human sperm and diploid cells. Nature, 1988; 335: 414-417.<\/li>\n<li>Handyside AH, et al. Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification. Nature, 1990; 344: 768-770.<\/li>\n<li>Wells D, et al. Detailed chromosomal and molecular genetic analysis of single cells by whole genome amplification and comparative genomic hybridisation. Nucleic Acids Res, 1999; 27: 1.214-1.218.<\/li>\n<li>Fiorentino F., et al. Development and validation of a next-generation sequencing-based protocol for 24-chromosome aneuploidy screening of embryos. Fertil Steril, 2014; 101: 1.375-1.382.<\/li>\n<li>Handyside AH. 24-chromosome copy number analysis: a comparison of available technologies. Fertil Steril, 2013; 100: 595-602.<\/li>\n<li>Scott RT Jr, et al. Cleavage-stage biopsy significantly impairs human embryonic implantation potential while blastocyst biopsy does not: a randomized and paired clinical trial. Fertil Steril, 2013; 100: 624-630.<\/li>\n<li>Liu W, et al. Non-invasive pre-implantation aneuploidy screening and diagnosis of beta thalassemia IVSII654 mutation using spent embryo culture medium. Ann Med, 2017; 49: 319-328.<\/li>\n<li>Vera-Rodriguez M, et al. Origin and composition of cell-free DNA in spent medium from human embryo culture during preimplantation development. Hum Reprod, 2018; 33: 745-756.<\/li>\n<li>Hammond ER, Shelling AN, Cree LM. Nuclear and mitochondrial DNA in blastocoele fluid and embryo culture medium: evidence and potential clinical use. Hum Reprod, 2016; 31: 1.653-1.661.<\/li>\n<li>De Rycke M, et al. ESHRE PGD Consortium data collection XIV-XV: cycles from January 2011 to December 2012 with pregnancy follow-up to October 2013. Hum Reprod, 2017; 32: 1.974-1.994.<\/li>\n<li>Dahdouh EM, Balayla J, Garcia-Velasco JA. Comprehensive chromosome screening improves embryo selection: a meta-analysis. Fertil Steril, 2015; 104: 1.503-1.512.<\/li>\n<li>Munne S, et al. Detailed investigation into the cytogenetic constitution and pregnancy outcome of replacing mosaic blastocysts detected with the use of high-resolution next-generation sequencing. Fertil Steril, 2017; 108: 62-71 e8.<\/li>\n<li>Munne S, Wells D. Detection of mosaicism at blastocyst stage with the use of high-resolution next-generation sequencing. Fertil Steril, 2017; 107: 1.085-1.091.<\/li>\n<li>Verpoest W, et al. Preimplantation genetic testing for aneuploidy by microarray analysis of polar bodies in advanced maternal age: a randomized clinical trial. Hum Reprod, 2018; 33: 1.767-1.776.<\/li>\n<li>Practise Committees of the American Society for Reproductive Medicine and the Society for Assisted Reproductive Technology. The use of preimplantation genetic testing for aneuploidy (PGT-A): a committee opinion. Fertil Steril, 2018; 109: 429-436.<\/li>\n<\/ol>\n<\/div><div class=\"fusion-clearfix\"><\/div><\/div><\/div><\/div><\/div><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-2 fusion-parallax-fixed nonhundred-percent-fullwidth non-hundred-percent-height-scrolling fusion-equal-height-columns\" style=\"background-color: rgba(255,255,255,0);background-image: url(&quot;http:\/\/www.iasoivf.com\/wp-content\/uploads\/2015\/12\/make-an-appointment.jpg&quot;);background-position: center center;background-repeat: no-repeat;padding-top:110px;padding-right:0px;padding-bottom:100px;padding-left:0px;margin-bottom: 0px;margin-top: 0px;border-width: 0px 0px 0px 0px;border-color:#eae9e9;border-style:solid;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;background-attachment:fixed;\" ><div class=\"fusion-builder-row fusion-row\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-1 fusion_builder_column_3_5 3_5 fusion-three-fifth fusion-column-first\" style=\"width:58.4%; margin-right: 4%;margin-top:0px;margin-bottom:30px;\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><div class=\"fusion-text fusion-text-2\"><h2 style=\"color: #ffffff; text-align: left;\">Make An Appointment<\/h2>\n<\/div><div class=\"fusion-text fusion-text-3\"><h3 style=\"color: #fcfcfc; text-align: left;\">Schedule your appointment today.<\/h3>\n<\/div><div class=\"fusion-clearfix\"><\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-2 fusion_builder_column_2_5 2_5 fusion-two-fifth fusion-column-last\" style=\"width:37.6%;margin-top:0px;margin-bottom:30px;\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><div class=\"fusion-column-content-centered\"><div class=\"fusion-column-content\"><div class=\"fusion-align-block\"><style type=\"text\/css\">.fusion-button.button-1 {border-radius:2px;}<\/style><a class=\"fusion-button button-flat button-xlarge button-default button-1 fusion-button-default-span fusion-button-default-type\" target=\"_self\" href=\"http:\/\/www.iasoivf.com\/contact-us\/\"><span class=\"fusion-button-text\">CONTACT US<\/span><\/a><\/div><\/div><\/div><div class=\"fusion-clearfix\"><\/div><\/div><\/div><\/div><\/div><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":6,"featured_media":3373,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[59],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.5 - 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